|
KMID : 0613820070170050671
|
|
Journal of Life Science
2007 Volume.17 No. 5 p.671 ~ p.677
|
|
|
Effects of Water Extract from Fermented Chaga Mushroom (Inonotus obliquus) on the Proliferation of Human Cancer Cell Lines
|
|
Cha Jae-Young
Park Sang-Hyun
Huo Jin-Sun
Cho Young-Su
|
|
|
Abstract
|
|
|
|
This study was performed to investigate the effect of the water-extract from non-fermented or fermented Chaga mushrooms (Inonotus obliquus) on the proliferation and apoptosis of the NIH3T3 mouse normal fibroblast cells and various human cancer cell lines including HCT-15 human colon carcinoma, AGS human gastric carcinoma, MCF-7 human breast adenocarcinoma, Hep3B human hepatocellular carcinoma and HeLa human cervical carcinoma using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay and DNA fragmentation. In an anti-cancer test using various human cancer cells, fermented Chaga mushroom extract showed higher antiproliferating effect than that of non-fermented Chaga mushroom extract. Mouse normal NIH3T3 cells were exhibited 80% above survival under fermented or non-fermented Chaga mushroom extract of various concentrations (0, 0.5 and 1㎎/㎖). Fermented Chaga mushroom extract significantly inhibited cell growth on HCT-15 cells in a dose-dependent manner. HCT-15 cells treated with non-fermented or fermented Chaga mushrooms extract produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis. These results suggest that fermented Chaga mushroom extract suppresses growth of HCT-15 human colon carcinoma cells through apoptosis.
|
|
|
KEYWORD
|
|
|
Inonoius abliquus, NIH3T3, HCT-15, AGS, MCF-7, Hep3B, HeLa
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|